This site reproduces the NHLBI mpkCCD Transcriptome Database with a purpose to speed up the access. Permission for the reproduction was granted by the database owner Mark A. Knepper MD, PhD.
This database of murine immortalized cortical collecting duct cell (mpkCCD) transcripts is based on Affymetrix microarray profiling carried out in the Laboratory of Kidney and Electrolyte Metabolism, NHLBI. Use the menu bar above to sort the database by different characteristics.
Five individual mpkCCD cell lines with various aquaporin 2 (AQP2) protein expression levels were cloned from the original mpkCCDc14 cell line. Transcripts with signal intensity above background are listed. Values are median-normalized signal intensity for the transcripts. Only values from clone 11 that has the highest AQP2 expression level are shown. Pearson’s correlation coefficients between the transcript abundance and AQP2 transcript abundance among the original and the 5 clones are shown. Significance of correlation, * p<0.05, ** p<0.01, *** p<0.005. ns, not significant.
These experimental data are derived from 6 GeneChip® Mouse Genome 430 2.0 Arrays by Affymetrix Inc. Data can be obtained via the Download Data link on this page.
The mpkCCD Transcriptome Database was reproduced by Ming-Jiun Yu, PhD.
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