This site reproduces the NHLBI mpkCCD Proteome Database with a purpose to speed up the access. Permission for the reproduction was granted by the database owner Mark A. Knepper MD, PhD.
The mpkCCD Cell Phosphoproteomic Database (mCPD) contains protein mass spectrometry data from the NHLBI Laboratory of Kidney and Electrolyte Metabolism. All data are obtained from a clonal mpkCCD cell line "clone 11" derived from mouse cortical collecting duct. Experiment description: The cells were grown on membrane supports until confluence and treated with the vasopessin V2 receptor specific agonist dDAVP for 3 days (1nM). After withdrawal of the hormone for 6h, cells were treated with dDAVP 0.1nM or vehicle for 30 min. All experiments were conducted using Stable Isotope Labeling by Amino-Acids in Cell culture (SILAC), which enables quantification of the hormone effect for a large number of peptides. If available, the ratio log2(dDAVP/vehicle) is given for the corresponding peptide. For more details, see the method section of the current paper (the reference will be added here upon publication of the manuscript).
The mpkCCD Phosphoproteome Database was reproduced by Ming-Jiun Yu, PhD.
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