This database lists interacting proteins of HCV hypo- (p56) and hyper-phosphorylated (p58) NS5A in the HCV J6/JFH1-infected Huh7.5.1 cells. Experiments were done in the Systems Biology of Epithelia Laboratory in the Institute of Biochemistry and Molecular Biology, College of Medicine, National Taiwan University. p56 (or p58) and the interacting proteins were co-immunoprecipitated with a custom-made p56 (or p58) specific antibody followed by dimethyl labeling-based quantitative proteomics (LTQ-Orbitrap-velos). The p56 fraction was labeled with light dimethyl group whereas the p58 fraction was labeled with heavy dimethyl group. Labeling-based protein quantification was performed with the MaxQuant software (version 1.5.5.1). Only unique peptides were used for quantification. Protein identification were achieved with the Andromeda software against an HCV J6/JFH1 database (UniprotKB, UP000008096), a human database (UniprotKB, UP000005640). False positive discovery rate was set at < 1% using reversed sequences as the decoy database.

Number of protein: 629 (download).

This webpage was established by Ting-Chun Pan. Contact for questions and comments: Ming-Jiun Yu.

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